Coding
Part:BBa_K1403016:Design
Designed by: Paris Bettencourt 2014 Group: iGEM14_Paris_Bettencourt (2014-10-06)
aldB CDS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We got the original L.Lactis aldB sequence from NCBI database. The sequence codes for acetolactate decarboxylase activity that transforms acetolactate produced by ilvBN gene, that original exists in E.Coli strain, to Acetoin, which produces butter smell. We then optimized the sequence. It is used for the production of butter smell for Paris-Bettencourt 2014 odor library project. It was transformed with the plasmid double expressing ilvBN gene, in order to raise basal metabolism of the pathway.
Source
L. lactis Sequence from NCBI database
References
David R. Nielsen, Sang-Hwal Yoon, Clara J. Yuan and Kristala L. J. Prather, Metabolic engineering of acetoin and meso-2,3-butanediol biosynthesis in E. coli, January 2010.